primary antibodies against neun for neurons  (Synaptic Systems)

Journal: CNS Neuroscience & Therapeutics

Article Title: Alleviating sleep disturbances and modulating neuronal activity after ischemia: Evidence for the benefits of zolpidem in stroke recovery

doi: 10.1111/cns.14637

Figure Lengend Snippet: c‐Fos and NeuN immunostaining in adjacent brain slices at striatum level reveal neuronal loss in the ischemia‐involved region of rats with 30‐min middle cerebral artery occlusion (MCAO) following 24 h reperfusion. (A–C) Representative images of c‐Fos immunostaining (black) from a coronal section at the level of the striatum. The black dotted lines indicate the infarct boundary. The boxed regions in (A) are enlarged in (B) and (C), showing the cortex and striatal ischemic border zone after MCAO, respectively. (D–F) Representative images of NeuN immunostaining (brown) from an adjacent brain slice of (A), NeuN‐expression (brown color) was significantly reduced in the infarct area. The black dotted lines indicate the infarct boundary. The boxed regions in (D) are enlarged in (E) and (F), showing the cortex and striatal ischemic border zone after MCAO, respectively. Note that the infarcts elucidated by c‐Fos and NeuN immunostaining are very similar. n = 4. CPu, caudate putamen; gcc, genu of the corpus callosum; LV, lateral ventricle.

Article Snippet: Sections of the striatum from another series of sections were stained with a rabbit polyclonal primary antibody against neuronal nuclei (NeuN, 1:2000, ABN78, Millipore) to value the ischemic neuronal injury, and the process was the same as the c‐Fos staining except that the peroxidase reaction was not strengthened.

Techniques: Immunostaining, Slice Preparation, Expressing